![BioTek Synergy™ H1m](/sites/unl.edu.cehs.biomedical-obesity-research-core/files/media/image/Biotek_0.jpg)
Synergy™ H1m is a flexible monochromator-based multi-mode microplate reader. The monochromator optics use a third generation quadruple grating design that works at any excitation or emission wavelength with a 1 nm step. This system supports top and bottom fluorescence intensity, UV-visible absorbance and high performance luminescence detection. It is the ideal system for all the standard microplate applications found in life science research laboratories. It has the following features:
- A dual reagent injection system is available to automate inject/read assays such as ion channels assays or flash luminescence assays (e.g. luciferase or ATP assays).
- User-adjustable orbital shaking and advanced 4-Zone™ temperature control
- Compatible with Take3™ Micro-Volume Plates: Samples down to 2 μL volume can be measured. Especially useful when working with precious samples, for fast and accurate DNA/RNA quantification at 260 nm
Video Introduction from The Lab World Group
GENERAL | |
Detection modes | UV-Vis absorbance |
Fluorescence intensity | |
Luminescence | |
Fluorescence polarization | |
Time-resolved fluorescence | |
Read methods | Endpoint, kinetic, spectral scanning, well area scanning |
Microplate types | 6- to 384-well plates |
Other labware supported | Petri and cell culture dishes |
Take3 Micro-Volume Plates | |
Temperature control | 4-Zone™ incubation to 45 °C with Condensation Control™ |
+0.2 °C at 37 °C | |
Shaking | Linear, orbital, double orbital |
Software | Gen5™ Data Analysis Software |
Gen5 Secure for 21 CFR Part 11 compliance (option) | |
Automation | BioStack and 3rd party automation compatible |
BioSpa 8 Automated Incubator compatible | |
CO2 and O2 control (option) | Range: 0 - 20% (CO2); 1 - 19% (O2) |
Control Resolution: +0.1% (CO2 and O2) | |
Stability: +0.2% at 5% CO2; +0.2% at 1% O2 | |
Models for both CO2 and O2 or CO2 only are available | |
ABSORBANCE | |
Light source | Xenon flash |
Detector | photodiode |
Wavelength selection | monochromator |
Wavelength range | 230 - 999 nm, 1 nm increments |
Monochromator bandwidth | 4 nm (230-285 nm), 8 nm (>285 nm) |
Dynamic range | 0 - 4.0 OD |
Resolution | 0.0001 OD |
Pathlength correction | yes |
Monochromator wavelength accuracy | ±2 nm |
Monochromator wavelength repeatability | ±0.2 nm |
OD accuracy | <1% at 2.0 OD |
<3% at 3.0 OD | |
OD linearity | <1% from 0 to 3.0 OD |
OD repeatability | <0.5% at 2.0 OD |
Stray light | 0.03% at 230 nm |
Reading speed (kinetic) | 96 wells: 11 seconds |
384 wells: 22 seconds | |
FLUORESCENCE INTENSITY | |
Light source | Xenon flash |
Detector | PMT for monochromator system |
PMT for filter system | |
Wavelength selection | Quad monochromators (top/bottom) |
Filters (top) | |
Wavelength range | Monochromators: 250 - 700 nm (850 nm option) |
Filters: 200 - 700 nm (850 nm option) | |
Monochromator bandwidth | Fixed, 16 nm |
Dynamic range | 7 decades |
Sensitivity | Filters: |
Fluorescein 0.25 pM (0.025 fmol/well, 384-well plate) | |
Quad Monochromator: | |
Fluorescein 2.5 pM (0.25 fmol/well, 384-well plate) - top | |
Fluorescein 4 pM (0.4 fmol/well, 384-well plate) - bottom | |
Reading speed (kinetic) | 96 wells: 11 seconds |
384 wells: 22 seconds | |
LUMINESCENCE | |
Wavelength range | 300 - 700 nm |
Dynamic range | >6 decades |
Sensitivity | Monos: 20 amol ATP (flash) |
Filters: 10 amol ATP (flash), 100 amol (glow) | |
FLUORESCENCE POLARIZATION | |
Light source | Xenon flash |
Detector | PMT |
Wavelength selection | Filters |
Wavelength range | 280 - 700 nm (850 nm option) |
Sensitivity | 1.2 mP standard deviation at 1 nm fluorescein |
TIME-RESOLVED FLUORESCENCE | |
Light source | Xenon flash |
Detector | PMT |
Wavelength selection | Quad monochromators (secondary mode) |
Filters (top) | |
Wavelength range | Filters: 200 - 700 nm (850 nm option) |
Sensitivity | Filters: Europium 40 fM (4 amol/well, 384-well plate) |
Monos: Europium 1200 fM (120 amol/well, 384-well plate) | |
REAGENT DISPENSERS | |
Supported detection modes | All modes |
Number | 2 syringe pumps |
Supported labware | 6- to 384-well microplates, Petri dishes |
Dead volume | 1.1 mL with back flush |
Dispense volume | 5 - 1000 µL in 1 µL increment |
Dispense accuracy | ±1 µL or 2% |
Dispense precision | <2% at 50-200 µL |
PHYSICAL CHARACTERISTICS | |
Power | 130 Watts max. |
Dimensions | 15.4"W 18.6"D 12.9"H |
(39.1 x 47.2 x 32.8 cm) | |
Weight | 50 lbs (22.5 kg) |